DeltaFlex TCSPC/MCS Fluorescence Lifetime System - HORIBA
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DeltaFlex

TCSPC/MCS Fluorescence Lifetime System

Ultimate TCSPC/MCS Performance and Flexibility for Lifetimes From 5 Picoseconds to Seconds

The HORIBA DeltaFlex™ system is the Time-Correlated Single Photon Counting (TCSPC) system of choice for the measurement of fluorescence lifetimes from picoseconds to seconds. Taking advantage of more than 45 years of TCSPC innovation, it is a complete fluorescence/phosphorescence system designed and optimized with innovative pulsed lasers and LEDs, timing electronics, detectors and sample handling components, all powered by HORIBA’s highly intuitive and extremely powerful EzTime™ touchscreen software interface. 

Segmento: Scientific
Fabricante: HORIBA Scientific

Unique Benefits from a Lifetime in Fluorescence

  • Flexibility to meet any requirement
  • Better user experience with automated component recognition and control
  • Extensive choice of sources and detectors to meet all wavelength and lifetime requirements
  • EzTime intuitive, automated, touchscreen software


DeltaFlex: Flexibility is in the name, and flexibility is in the design

The modular DeltaFlex system is comprised of a choice of the following main components:

  • Optical configuration
    Choose from filters or monochromators and single detector (L format) or simultaneous dual detector (T Format)
  • Excitation sources
    Choose from a plethora of pulsed lasers and LED sources
  • Detection modules
    Choose from a wide range of dedicated TCSPC detectors depending on your emission wavelength range, lifetime requirements and budget
  • Timing electronics
    Choose from standard or high resolution electronic interfaces depending on your lifetime requirements

 

All DeltaFlex systems are controlled using EzTime software which provides for full instrumentation control and acquisition, as well as a comprehensive suite of data analysis modules for fluorescence and phosphorescence lifetime determination, decay associated spectra (DAS), time-resolved anisotropy and uncorrected steady state emission spectra (if equipped with a scanning emission monochromator).

Optional Pulsed Laser and LED Light Sources

 

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                    scrollable

                     

                    DeltaDiode™ General Specifications

                    DeltaDiode TypePulse DurationRepetition RateAvailable Wavelengths
                    DeltaDiode Laser
                    (Denoted with an “L” on part number)
                    35 to 200 ps10 kHz to 100 MHz375 to 1,310 nm
                    DeltaDiode LED
                    (No “L” on part number)
                    750 to 950 ps10 kHz to 25 MHz265 to 455 nm
                    scrollable

                     

                    DeltaDiode Controller Specifications

                    DD-C1 Controller

                    FunctionSpecification

                    Repetition Rates

                    10 kHz, 20 kHz, 50 kHz, 100 kHz, 250 kHz, 500 kHz, 1 MHz, 2 MHz, 4 MHz, 5 MHz, 8 MHz, 10 MHz, 16 MHz, 20 MHz, 25 MHz, 50 MHz, 80 MHz, 100 MHz, or Trigger Input (single-shot to 50 MHz). Subject to attached head

                    Trigger Input

                    Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω, 20 ns minimum spacing

                    Sync Outputs

                    Simultaneous output of NIM-compatible (-0.8 Vpp 50 Ω) and TTL-compatible (+2 Vpp 50 Ω), automatic width selection 4-15 ns nominal

                    Sync Delay Control

                    Adjustment of sync output pulse timing in range -10 ns to +10 ns nominal in 1 ns steps, uncalibrated

                    Fast Gate Input

                    Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω. Selectable Inhibit/Enable modes

                    Slow Gate Input

                    Pulse amplitude +2 V to +5 V. 10 kΩ. Selectable Inhibit/enable modes. Operates in Pulsed and CW modes

                    Interlock

                    2-pin connector (included). Contacts must be short-circuited to enable emission

                    Connection to Head

                    1.5 m cable (included)

                    User Interface

                    LCD display (stand-alone operation) or software (PC control)

                    PC interface

                    USB 2.0 with integral hub for downstream connection to other USB peripherals (cable to host PC and software supplied)

                    Power Requirement

                    90 V to 250 V AC, 50/60 Hz, 100 VA

                    Operating Temperature

                    +15˚ C to +30˚ C (ambient)

                    Weight & Dimensions

                    3.1 kg, 234 x 255 x 92 mm

                    scrollable

                     

                    Solas MOFA Fiber Laser Specifications

                    Parameter

                    Solas 355L

                    Solas 532L

                    Solas 1064L

                    Power

                    > 7 mW at 80 MHz

                    > 30 mW at 80 MHz

                    > 300 mW at 80 MHz

                    Power Stability

                    0.5% RMS over 10 min

                    Pulse duration

                    80 to 100 ps (FWHM)

                    Spectral Width

                    < 0.2 nm

                    < 0.4 nm

                    Resolution Rate

                    Pulse on demand to 100 MHz

                    Pointing Stability

                    < 1 μrad

                    Polarization Extinction Ratio

                    > 20 dB

                    Beam Quality

                    Multi-mode fiber output

                    Circular TEM00 beam

                    scrollable

                     

                    SpectraLED Specifications

                     Pulse DurationRepetition RateAvailable Wavelengths
                    SpectraLED< 250 ns to > 10 s0.1 to 100 kHz265 to 1,275 nm
                    scrollable

                     

                    SpectraXE Specifications

                     Pulse DurationRepetition RateAvailable Wavelengths
                    SpectraXE0.4 μs0.1 Hz to 80 Hz185 to 2,000 nm
                    scrollable

                     

                    HPPD Specifications

                    ModelWavelength RangeTemporal Response
                    (IRF FWHM)
                    Dark CountQuantum Efficiency
                    HPPD-650220 to 650 nm50 ps< 100 cps28% (340 nm)
                    HPPD-720300 to 720 nm120 ps< 1000 cps47% (530 nm)
                    HPPD-860220 to 860 nm50 ps< 200 cps23% (280 nm)
                    HPPD-870300 to 870 nm130 ps< 500 cps26% (630 nm)
                    HPPD-890380 to 890 nm160 ps< 1000 cps16% (630 nm)
                    scrollable

                     

                    MCP-PMT Detectors

                     HPPD-860 COOLEDMCP-PMT (R3809-50)

                    Typical IRF FWHM at 400 nm

                    40-45 ps 

                    40-45 ps

                    Shortest Measurable Lifetime 

                    40-45 ps 

                    5 ps

                    Wavelength Response

                     220-860 nm

                    160-850 nm

                    Robust for Steady State Spectra

                    Yes

                    No

                    Amplifier + CFD

                    Integrated (no cable)

                    External

                    High Voltage Bias

                    Integrated (no cable)

                    External

                    Compatible with Phos and Steady State

                    Yes

                    No (requires second detector)

                    Temperature Control

                    Integrated TEC (air-cooled)

                    External (water-cooled)

                    Dark Count Rate (Cooled)

                     < 200 cps 

                     < 20 cps

                    PC Interface

                    N/A

                    N/A

                    scrollable

                     

                    NIR TCSPC Specifications

                     

                    ModelSensorWavelength RangeTemporal ResponseDark CountCooling

                    NIR-R4

                    R5509-43 PMT

                    300 to 1,400 nm

                    1.5 ns (TTS)

                    < 25,000 cps

                    Liquid nitrogen

                    NIR-R7

                    R5509-73 PMT

                    300 to 1,700 nm

                    1.5 ns (TTS)

                    < 250,000 cps

                    Liquid nitrogen

                    NIR-H2

                    H10330-25 PMT

                    950 to 1,200 nm

                    400 ps (TTS)

                    < 2,500 cps

                    Thermo-electric

                    NIR-H4

                    H10330-45 PMT

                    950 to 1,400 nm

                    400 ps (TTS)

                    < 25,000 cps

                    Thermo-electric

                    NIR-H7

                    H10330-75 PMT

                    950 to 1,700 nm

                    400 ps (TTS)

                    < 250,000 cps

                    Thermo-electric

                    NIR-S1

                    Count-100N SPAD

                    400 to 1,000 nm

                    < 3 ns (TTS)

                    100 cps

                    None

                    scrollable

                     

                    FiPho TCSPC Electronics Specifications

                    Specifications

                    FiPho

                    FiPho-HR

                    Full Detectable TCSPC Lifetime Range

                    <20 ps to 30 sec

                    5 ps to 30 sec

                    TCSPC Converter Type

                    Digital TDC

                    Digital TDC and Analog TAC

                    TCSPC Bin Width

                    <15 ps

                    ~ 250 fs

                    Phosphorescence Mode

                    MCS

                    MCS

                    Independent Stop Channels

                    1 to 4

                    1 to 4

                    Photon Streaming

                    Included

                    Included

                    FLIM Capable

                    Yes

                    Yes

                    scrollable

                     

                    Detailed FiPho Electronics Specifications

                    Specifications

                    FiPho (TDC, MCS)

                    FiPho-HR (TDC, TAC, MCS)

                    Full Detectable Lifetime Range

                    <20 ps to 30 sec

                    5 ps to 30 sec

                    TCSPC Time Range

                    <2 ns to 55 μs

                    <2 ns to 55 μs

                    Deadtime

                    5 ns

                    5 ns

                    TCSPC Bin Width

                    <15 ps

                    ~ 250 fs

                    Electronics Jitter (FWHM)

                    30 ps

                    < 10 ps

                    TCSPC Histogram Size

                    Up to 16k

                    Up to 64k

                    Histogram Bin Depth

                    32 bit

                    32 bit

                    Independent Stops

                    1 to 4

                    1 to 4

                    Maximum Start Rate

                    100 MHz

                    100 MHz

                    Maximum Stop Rate

                    40 Mcps

                    40 Mcps

                    Operating Mode

                    Automatic Forward Timing

                    Automatic Forward or Reverse timing

                    Streaming Mode

                    Photon Streaming (Time-Tag)

                    Photon Streaming (Time-Tag)

                    MCS Bin Width

                    5 ns

                    5 ns

                    MCS Time Range

                    < 2.5 μs to 330 seconds

                    < 2.5 μs to 330 seconds

                    Maximum MCS Histogram Size

                    64k

                    64k

                    Acquisition and Analysis Macro Scripting

                    Yes

                    Yes

                    PC Interface

                    USB 3.0

                    USB 3.0

                    Software

                    EzTime, EzTime Image

                    EzTime, EzTime Image

                    scrollable

                    HORIBA Scientific has a policy of continuous product development, and reserves the right to amend part numbers, descriptions and specifications without prior notice.

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                    Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
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                    Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
                    Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
                    Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. Reactants are expelled from syringes, mixed and injected into a flowcell.
                    Fluorescence Anisotropy Studies
                    Fluorescence Anisotropy Studies
                    Polarized light striking a fluorescent molecule results in polarized fluorescence. This polarized emission gradually returns to unpolarized fluorescence depending on rotational diffusion and other factors. Anisotropy is directly related to the polarization, and is the ratio of the polarized light component to the total light intensity.
                    Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
                    Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
                    Upconverting lanthanide-based nanomaterials exhibit a unique fluorescence anti-Stokes shift, which enables them to convert NIR wavelength excitation into visible shorter wavelength emissions (NIR to UV-Vis).
                    Characterizing Lanthanides in Glasses for Optical Applications
                    Characterizing Lanthanides in Glasses for Optical Applications
                    Glasses are essential materials with a multitude of uses and many forms. In the area of optoelectronics there is an interest to modify the glass composition to favor the incorporation of lanthanide elements.
                    Upconversion of Lanthanide-containing glasses using DD‐980L excitation
                    Upconversion of Lanthanide-containing glasses using DD‐980L excitation
                    The phenomenon of upconversion is an optical process that takes in lower energy (longer wavelength) photons and emits higher energy (shorter wavelength) photons.
                    Measurement of carrier lifetime in perovskite for solar cell applications
                    Measurement of carrier lifetime in perovskite for solar cell applications
                    Hybrid perovskite photovoltaics (PV) show promise because of their good efficiencies, which can be around 20%. Along with their PV characteristics, perovskite materials exhibit a high degree of radiative recombination.
                    Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
                    Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
                    Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process.
                    The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
                    The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
                    The study of singlet oxygen (1O2) is of interest, principally, as it is a highly reactive species. It can be produced by photosensitisation, usually of a molecule such as a dye or porphyrin. Thus, by the appropriate selection of sensitiser, the presence of oxygen and light, 1O2 can be selectively generated. From a biological aspect it has the ability to damage and destroy cells, which has lead to interest in its use as an anticancer agent in photodynamic therapy (PDT).
                    Effect of temperature on HSA structure inferred using time-resolved room-temperature phosphorescence
                    Effect of temperature on HSA structure inferred using time-resolved room-temperature phosphorescence
                    To access intrinsic amino acids, such as tryptophan, as probes, the UV excitation wavelengths for pulsed phosphorescence measurements have long been the preserve of low-repetition-rate gas-filled lamps or larger laser systems. Recent developments have enabled the use of interchangeable semiconductor diodes...
                    Plasmon enhancement of protein fluorescence by silver nanostructures
                    Plasmon enhancement of protein fluorescence by silver nanostructures
                    The use of metal surfaces in conjunction with fluorescence molecules employing a plasmon effect, sometimes referred to as metal enhanced fluorescence, can be advantageous because of the possible enhancement of photophysical properties.
                    Investigating photocleavage using time‐resolved emission spectra
                    Investigating photocleavage using time‐resolved emission spectra
                    The choice of protecting group is of crucial importance in the success of many steps in organic synthesis and the manipulation of polyfunctional molecules, since they can prevent the formation of undesired side products and reactions.
                    Time‐resolved luminescence of security inks from the UV to NIR
                    Time‐resolved luminescence of security inks from the UV to NIR
                    The use of security features, such as luminescent inks, has increased significantly in an attempt to prevent fraud and counterfeiting of materials and goods.
                    Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
                    Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
                    Certain fluorescent molecules, known as molecular rotors, can be employed to estimate the local (nanoscale) viscosity in microheterogeneous systems by measurement of their fluorescence lifetime. This can be advantageous over the usual fluorescence anisotropy method, as the measurement is simpler and faster to perform. This is demonstrated using the HORIBA Scientific TemPro fluorescence lifetime system to monitor the gelation of silica produced using the sol‐gel technique.
                    MCS and Protein Phosphorescence
                    MCS and Protein Phosphorescence
                    Tryptophan phosphorescence within protein molecules is gaining attention as a probe of protein dynamics and structure. The tryptophan phosphorescence lifetime, τ, varies with the protein molecule’s local environment and conformation.

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