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DeltaFlex

TCSPC/MCS Fluorescence Lifetime System

Ultimate TCSPC/MCS Performance and Flexibility for Lifetimes From 5 Picoseconds to Seconds

The HORIBA DeltaFlex™ system is the Time-Correlated Single Photon Counting (TCSPC) system of choice for the measurement of fluorescence lifetimes from picoseconds to seconds. Taking advantage of more than 45 years of TCSPC innovation, it is a complete fluorescence/phosphorescence system designed and optimized with innovative pulsed lasers and LEDs, timing electronics, detectors and sample handling components, all powered by HORIBA’s highly intuitive and extremely powerful EzTime™ touchscreen software interface. 

Сегмент: Scientific
Подразделение: Fluorescence Spectroscopy
Производитель: HORIBA Scientific

Unique Benefits from a Lifetime in Fluorescence

  • Flexibility to meet any requirement
  • Better user experience with automated component recognition and control
  • Extensive choice of sources and detectors to meet all wavelength and lifetime requirements
  • EzTime intuitive, automated, touchscreen software


DeltaFlex: Flexibility is in the name, and flexibility is in the design

The modular DeltaFlex system is comprised of a choice of the following main components:

  • Optical configuration
    Choose from filters or monochromators and single detector (L format) or simultaneous dual detector (T Format)
  • Excitation sources
    Choose from a plethora of pulsed lasers and LED sources
  • Detection modules
    Choose from a wide range of dedicated TCSPC detectors depending on your emission wavelength range, lifetime requirements and budget
  • Timing electronics
    Choose from standard or high resolution electronic interfaces depending on your lifetime requirements

 

All DeltaFlex systems are controlled using EzTime software which provides for full instrumentation control and acquisition, as well as a comprehensive suite of data analysis modules for fluorescence and phosphorescence lifetime determination, decay associated spectra (DAS), time-resolved anisotropy and uncorrected steady state emission spectra (if equipped with a scanning emission monochromator).

Optional Pulsed Laser and LED Light Sources

 

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                  scrollable

                   

                  DeltaDiode™ General Specifications

                  DeltaDiode TypePulse DurationRepetition RateAvailable Wavelengths
                  DeltaDiode Laser
                  (Denoted with an “L” on part number)
                  35 to 200 ps10 kHz to 100 MHz375 to 1,310 nm
                  DeltaDiode LED
                  (No “L” on part number)
                  750 to 950 ps10 kHz to 25 MHz265 to 455 nm
                  scrollable

                   

                  DeltaDiode Controller Specifications

                  DD-C1 Controller

                  FunctionSpecification

                  Repetition Rates

                  10 kHz, 20 kHz, 50 kHz, 100 kHz, 250 kHz, 500 kHz, 1 MHz, 2 MHz, 4 MHz, 5 MHz, 8 MHz, 10 MHz, 16 MHz, 20 MHz, 25 MHz, 50 MHz, 80 MHz, 100 MHz, or Trigger Input (single-shot to 50 MHz). Subject to attached head

                  Trigger Input

                  Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω, 20 ns minimum spacing

                  Sync Outputs

                  Simultaneous output of NIM-compatible (-0.8 Vpp 50 Ω) and TTL-compatible (+2 Vpp 50 Ω), automatic width selection 4-15 ns nominal

                  Sync Delay Control

                  Adjustment of sync output pulse timing in range -10 ns to +10 ns nominal in 1 ns steps, uncalibrated

                  Fast Gate Input

                  Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω. Selectable Inhibit/Enable modes

                  Slow Gate Input

                  Pulse amplitude +2 V to +5 V. 10 kΩ. Selectable Inhibit/enable modes. Operates in Pulsed and CW modes

                  Interlock

                  2-pin connector (included). Contacts must be short-circuited to enable emission

                  Connection to Head

                  1.5 m cable (included)

                  User Interface

                  LCD display (stand-alone operation) or software (PC control)

                  PC interface

                  USB 2.0 with integral hub for downstream connection to other USB peripherals (cable to host PC and software supplied)

                  Power Requirement

                  90 V to 250 V AC, 50/60 Hz, 100 VA

                  Operating Temperature

                  +15˚ C to +30˚ C (ambient)

                  Weight & Dimensions

                  3.1 kg, 234 x 255 x 92 mm

                  scrollable

                   

                  Solas MOFA Fiber Laser Specifications

                  Parameter

                  Solas 355L

                  Solas 532L

                  Solas 1064L

                  Power

                  > 7 mW at 80 MHz

                  > 30 mW at 80 MHz

                  > 300 mW at 80 MHz

                  Power Stability

                  0.5% RMS over 10 min

                  Pulse duration

                  80 to 100 ps (FWHM)

                  Spectral Width

                  < 0.2 nm

                  < 0.4 nm

                  Resolution Rate

                  Pulse on demand to 100 MHz

                  Pointing Stability

                  < 1 μrad

                  Polarization Extinction Ratio

                  > 20 dB

                  Beam Quality

                  Multi-mode fiber output

                  Circular TEM00 beam

                  scrollable

                   

                  SpectraLED Specifications

                   Pulse DurationRepetition RateAvailable Wavelengths
                  SpectraLED< 250 ns to > 10 s0.1 to 100 kHz265 to 1,275 nm
                  scrollable

                   

                  SpectraXE Specifications

                   Pulse DurationRepetition RateAvailable Wavelengths
                  SpectraXE0.4 μs0.1 Hz to 80 Hz185 to 2,000 nm
                  scrollable

                   

                  HPPD Specifications

                  ModelWavelength RangeTemporal Response
                  (IRF FWHM)
                  Dark CountQuantum Efficiency
                  HPPD-650220 to 650 nm50 ps< 100 cps28% (340 nm)
                  HPPD-720300 to 720 nm120 ps< 1000 cps47% (530 nm)
                  HPPD-860220 to 860 nm50 ps< 200 cps23% (280 nm)
                  HPPD-870300 to 870 nm130 ps< 500 cps26% (630 nm)
                  HPPD-890380 to 890 nm160 ps< 1000 cps16% (630 nm)
                  scrollable

                   

                  MCP-PMT Detectors

                   HPPD-860 COOLEDMCP-PMT (R3809-50)

                  Typical IRF FWHM at 400 nm

                  40-45 ps 

                  40-45 ps

                  Shortest Measurable Lifetime 

                  40-45 ps 

                  5 ps

                  Wavelength Response

                   220-860 nm

                  160-850 nm

                  Robust for Steady State Spectra

                  Yes

                  No

                  Amplifier + CFD

                  Integrated (no cable)

                  External

                  High Voltage Bias

                  Integrated (no cable)

                  External

                  Compatible with Phos and Steady State

                  Yes

                  No (requires second detector)

                  Temperature Control

                  Integrated TEC (air-cooled)

                  External (water-cooled)

                  Dark Count Rate (Cooled)

                   < 200 cps 

                   < 20 cps

                  PC Interface

                  N/A

                  N/A

                  scrollable

                   

                  NIR TCSPC Specifications

                   

                  ModelSensorWavelength RangeTemporal ResponseDark CountCooling

                  NIR-R4

                  R5509-43 PMT

                  300 to 1,400 nm

                  1.5 ns (TTS)

                  < 25,000 cps

                  Liquid nitrogen

                  NIR-R7

                  R5509-73 PMT

                  300 to 1,700 nm

                  1.5 ns (TTS)

                  < 250,000 cps

                  Liquid nitrogen

                  NIR-H2

                  H10330-25 PMT

                  950 to 1,200 nm

                  400 ps (TTS)

                  < 2,500 cps

                  Thermo-electric

                  NIR-H4

                  H10330-45 PMT

                  950 to 1,400 nm

                  400 ps (TTS)

                  < 25,000 cps

                  Thermo-electric

                  NIR-H7

                  H10330-75 PMT

                  950 to 1,700 nm

                  400 ps (TTS)

                  < 250,000 cps

                  Thermo-electric

                  NIR-S1

                  Count-100N SPAD

                  400 to 1,000 nm

                  < 3 ns (TTS)

                  100 cps

                  None

                  scrollable

                   

                  FiPho TCSPC Electronics Specifications

                  Specifications

                  FiPho

                  FiPho-HR

                  Full Detectable TCSPC Lifetime Range

                  <20 ps to 30 sec

                  5 ps to 30 sec

                  TCSPC Converter Type

                  Digital TDC

                  Digital TDC and Analog TAC

                  TCSPC Bin Width

                  <15 ps

                  ~ 250 fs

                  Phosphorescence Mode

                  MCS

                  MCS

                  Independent Stop Channels

                  1 to 4

                  1 to 4

                  Photon Streaming

                  Included

                  Included

                  FLIM Capable

                  Yes

                  Yes

                  scrollable

                   

                  Detailed FiPho Electronics Specifications

                  Specifications

                  FiPho (TDC, MCS)

                  FiPho-HR (TDC, TAC, MCS)

                  Full Detectable Lifetime Range

                  <20 ps to 30 sec

                  5 ps to 30 sec

                  TCSPC Time Range

                  <2 ns to 55 μs

                  <2 ns to 55 μs

                  Deadtime

                  5 ns

                  5 ns

                  TCSPC Bin Width

                  <15 ps

                  ~ 250 fs

                  Electronics Jitter (FWHM)

                  30 ps

                  < 10 ps

                  TCSPC Histogram Size

                  Up to 16k

                  Up to 64k

                  Histogram Bin Depth

                  32 bit

                  32 bit

                  Independent Stops

                  1 to 4

                  1 to 4

                  Maximum Start Rate

                  100 MHz

                  100 MHz

                  Maximum Stop Rate

                  40 Mcps

                  40 Mcps

                  Operating Mode

                  Automatic Forward Timing

                  Automatic Forward or Reverse timing

                  Streaming Mode

                  Photon Streaming (Time-Tag)

                  Photon Streaming (Time-Tag)

                  MCS Bin Width

                  5 ns

                  5 ns

                  MCS Time Range

                  < 2.5 μs to 330 seconds

                  < 2.5 μs to 330 seconds

                  Maximum MCS Histogram Size

                  64k

                  64k

                  Acquisition and Analysis Macro Scripting

                  Yes

                  Yes

                  PC Interface

                  USB 3.0

                  USB 3.0

                  Software

                  EzTime, EzTime Image

                  EzTime, EzTime Image

                  scrollable

                  HORIBA Scientific has a policy of continuous product development, and reserves the right to amend part numbers, descriptions and specifications without prior notice.

                  Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
                  Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
                  The potential health benefits stemming from the antioxidant activity of curcumin, commonly found in turmeric (Curcuma longa L), has attracted the interest of several research groups.
                  Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
                  Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
                  Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. Reactants are expelled from syringes, mixed and injected into a flowcell.
                  Fluorescence Anisotropy Studies
                  Fluorescence Anisotropy Studies
                  Polarized light striking a fluorescent molecule results in polarized fluorescence. This polarized emission gradually returns to unpolarized fluorescence depending on rotational diffusion and other factors. Anisotropy is directly related to the polarization, and is the ratio of the polarized light component to the total light intensity.
                  Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
                  Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
                  Upconverting lanthanide-based nanomaterials exhibit a unique fluorescence anti-Stokes shift, which enables them to convert NIR wavelength excitation into visible shorter wavelength emissions (NIR to UV-Vis).
                  Characterizing Lanthanides in Glasses for Optical Applications
                  Characterizing Lanthanides in Glasses for Optical Applications
                  Glasses are essential materials with a multitude of uses and many forms. In the area of optoelectronics there is an interest to modify the glass composition to favor the incorporation of lanthanide elements.
                  Upconversion of Lanthanide-containing glasses using DD‐980L excitation
                  Upconversion of Lanthanide-containing glasses using DD‐980L excitation
                  The phenomenon of upconversion is an optical process that takes in lower energy (longer wavelength) photons and emits higher energy (shorter wavelength) photons.
                  Measurement of carrier lifetime in perovskite for solar cell applications
                  Measurement of carrier lifetime in perovskite for solar cell applications
                  Hybrid perovskite photovoltaics (PV) show promise because of their good efficiencies, which can be around 20%. Along with their PV characteristics, perovskite materials exhibit a high degree of radiative recombination.
                  Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
                  Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
                  Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process.
                  The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
                  The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
                  The study of singlet oxygen (1O2) is of interest, principally, as it is a highly reactive species. It can be produced by photosensitisation, usually of a molecule such as a dye or porphyrin. Thus, by the appropriate selection of sensitiser, the presence of oxygen and light, 1O2 can be selectively generated. From a biological aspect it has the ability to damage and destroy cells, which has lead to interest in its use as an anticancer agent in photodynamic therapy (PDT).
                  Effect of temperature on HSA structure inferred using time-resolved room-temperature phosphorescence
                  Effect of temperature on HSA structure inferred using time-resolved room-temperature phosphorescence
                  To access intrinsic amino acids, such as tryptophan, as probes, the UV excitation wavelengths for pulsed phosphorescence measurements have long been the preserve of low-repetition-rate gas-filled lamps or larger laser systems. Recent developments have enabled the use of interchangeable semiconductor diodes...
                  Plasmon enhancement of protein fluorescence by silver nanostructures
                  Plasmon enhancement of protein fluorescence by silver nanostructures
                  The use of metal surfaces in conjunction with fluorescence molecules employing a plasmon effect, sometimes referred to as metal enhanced fluorescence, can be advantageous because of the possible enhancement of photophysical properties.
                  Investigating photocleavage using time‐resolved emission spectra
                  Investigating photocleavage using time‐resolved emission spectra
                  The choice of protecting group is of crucial importance in the success of many steps in organic synthesis and the manipulation of polyfunctional molecules, since they can prevent the formation of undesired side products and reactions.
                  Time‐resolved luminescence of security inks from the UV to NIR
                  Time‐resolved luminescence of security inks from the UV to NIR
                  The use of security features, such as luminescent inks, has increased significantly in an attempt to prevent fraud and counterfeiting of materials and goods.
                  Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
                  Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
                  Certain fluorescent molecules, known as molecular rotors, can be employed to estimate the local (nanoscale) viscosity in microheterogeneous systems by measurement of their fluorescence lifetime. This can be advantageous over the usual fluorescence anisotropy method, as the measurement is simpler and faster to perform. This is demonstrated using the HORIBA Scientific TemPro fluorescence lifetime system to monitor the gelation of silica produced using the sol‐gel technique.
                  MCS and Protein Phosphorescence
                  MCS and Protein Phosphorescence
                  Tryptophan phosphorescence within protein molecules is gaining attention as a probe of protein dynamics and structure. The tryptophan phosphorescence lifetime, τ, varies with the protein molecule’s local environment and conformation.

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