Aqualog - Environmental Water Research Analyzer The Gold Standard for Water CDOM Research - HORIBA
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Aqualog - Environmental Water Research Analyzer
The Gold Standard for Water CDOM Research
수질 CDOM 연구를 위한 골드 스탠다드 EEM을 위한 스캐닝 형광계뿐만 아니라 CDOM(유색 용존 유기물)을 위한 훨씬 빠르고 우수한 A-TEEM 분광계 HORIBA Aqualog는 최고 표준 광학 분광기입니다.
색용존 유기물(CDOM) 연구를 위해 전 세계 환경 수질 연구에서 Aqualog는 형광 분광법을 사용하여 CDOM을 연구하는 전 세계 환경 수질 연구자의 요구를 충족하기 위해 발명되었습니다. 그 당시 연구자들은 여기-방출 매트릭스(EEM, excitation-emission matrix)라고 불리는 형광 여기 및 형광 방출 스펙트럼의 3차원 매트릭스를 천천히 얻기 위해 스캐닝 분광 형광계를 사용했습니다. EEM은 용해된 유기 물질을 연구하기 위한 fingerprint를 제공했으나, 단일 EEM 프로필을 수집하는 데 최대 1시간이 소요되어 연구원들은 하루 종일 실험실에 발이 붙잡혀있었습니다.
HORIBA Aqualog는 형광 EEM이 수집되는 속도를 크게 향상시키고 EEM 지문이 정량적인 동적 범위를 극적으로 증가시키며 동시에 물에 존재하는 비형광 분자의 흡광도 및 색상 분석을 위한 흡광도 스펙트럼을 획득합니다.
우리는 Aqualog가 사용하는 이 기술을 Absorbance-Transmission Excitation Emission Matrix 또는 A-TEEM™이라고 부릅니다. 오늘날 Aqualog는 가장 권위 있는 수질 연구실과 각종 지역에서 다양하고 중요한 연구 주제를 연구하는 데 사용됩니다.
CDOM(Color Dissolved Organic Matter) 및 그 파생물 DOM 및 NOM에 대한 최신 환경 연구 주제
Aqualog A-TEEM compared to traditional scanning PMT fluorometers
Traditional scanning spectrofluorometers have been used to collect a molecular fingerprint, in the form of a fluorescence excitation emission matrix, or EEM. Sometimes also referred to as 3D Fluorescence, an EEM is a three-dimensional data set of fluorescence excitation wavelength versus fluorescence emission wavelength versus fluorescence intensity. With a scanning spectrofluorometer, this data set is acquired by sequentially scanning a series of emission spectra, at varying excitation wavelengths, and then reconstructing the resultant data set three dimensionally. This three-dimensional data set can be used with third party multivariate analysis software for component analysis, as is done with other analytical techniques such as FTIR, HPLC and MS. There are, in fact, many scientific papers published citing the use of scanning spectrofluorometers for fluorescence EEM component analysis in many disciplines including food sciences, water research and pharmaceuticals.
There are, however, two fundamental limitations of using a traditional scanning PMT fluorometer for EEM component studies. The first is that it takes a very long time to collect a single EEM with a scanning fluorometer. Depending on the brightness of the signal, and the wavelength range and resolution that is required, a single EEM experiment can take a scanning spectrofluorometer up to an hour to collect!
Another important limitation of scanning fluorometers is that the shape of the fluorescence EEM fingerprint itself can change with even subtle variations in sample concentration. If an instrument measures different EEM fingerprints for the same molecule at different concentrations, it really can’t be used for component analysis. For an EEM to be used as a true analytical technique, the shape of the spectra must be independent of concentration.
These two inherent limitations of a scanning spectrofluorometer have impacted the usability of the fluorescence EEM technique, and this has lead to the development by HORIBA of the A-TEEM technique.
HORIBA’s unique A-TEEM technique overcomes these two limitations. With CCD detection technology, HORIBA solves the serious speed limitations of scanning spectrofluorometers because with HORIBA technology, an entire fluorescence EEM can be acquired in mere seconds to minutes depending on the sample.
Figure 1. Three dimensional contour plot viewed at an angle of a fluorescence EEM, with three dimensional axis for fluorescence excitation, emission and intensity.
Figure 2. Contour plot (top down view) of fluorescence EEM of fluorescein acquired in one second with Duetta.
HORIBA has also solved the problems associated with the fluorescence inner filter effect by taking advantage of the fact that the A-TEEM technique also collects absorbance of the same sample at the same time as the fluorescence, and uses the absorbance to correct EEMs for the inner filter effect (IFE).
HORIBA calls this technique A-TEEMTM, for Absorbance-Transmission Excitation Emission Matrix. By correcting for inner filter effects, the A-TEEM molecular fingerprint is a much more absolute representation of the true molecular fingerprint. Therefore when using third party multivariate chemometrics analysis software, the A-TEEM data provides much more robust component analysis than can be achieved with just a simple EEM from a scanning fluorometer.
Below is a good example to show how even a small concentration difference in a single molecule can have a significant effect on the shape of an EEM fingerprint, but with proper IFE correction an A-TEEM fingerprint remains the same.
Figure 3. Fluorescence Excitation Emission Matrices of two concentrations of quinine sulfate in tonic water diluted in 0.1 M perchloric acid (aq.) with and without inner-filter effect corrections applied. Acquired with HORIBA Duetta.
Aqualog A-TEEM chemometrics analysis presented here are derived from Eigenvector Research Incorporated, Solo software.
Spectral and kinetic analysis tools for both absorbance and fluorescence data
Methods and batch protocols for automating multiple sample measurement
NEW A-TEEM™ technology
A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!
All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.
Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.
NEW Aqualog® Datastream Dashboard
Features
Seamless integration with Aqualog
Convenient HTML-based interface
Push-button method operation
Simple administrator level controls for calibration and method development
Benefits
Easy access through internet or intranet
Dashboard shows the latest readings, time series and tables for trends and analysis
WTP can upload their own independent data
Aqualog Datastream Dashboard is powered by Solo_Predictor software from Eigenvector Research, Incorporated
NEW HORIBA Multi-Model Predictor Tool
HORIBA Instruments is pleased to introduce a new software tool designed to automate multivariate and machine learning analysis workflows for industrial QC/QA applications of HORIBA’s patented Aqualog Absorbance- Transmission fluorescence Excitation Emission Matrix (A-TEEM™) spectrometer.
Key Applications of HMMP Tool
Wine and Grape Quality Chemistry (phenolics, anthocyanins, tannins, sulfites etc..)
Water Contamination (oil, algae and other materials)
Grain mold and odor compounds
Cannabinoids
Pharmaceuticals (Drugs, Vaccines, Cell Media)
Olive Oil adulteration and spoilage
Dietary supplement adulteration
Key Features and Benefits
Easy, Rapid Operator Level Analysis
Facilitated Administration of Method Model Development and Editing
Complete Parameter Profile and Classification Reports
Compatible with Laboratory Information Management Systems
HMMP Add-In Fully Integrated into Eigenvector Inc. Solo/Solo+Mia and Exclusively Activated and Supported by HORIBA Instruments Inc.
NEW Automatic Sipper Accessory
New for our Aqualog® A-TEEM™ spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.
The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection. It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.
When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources.
NEW Fast-01 Autosampler Accessory
The Fast-01 can be configured to use a variety of sample-vials and racks to meet your application needs and enables complete temperature control. Sample vial repeats and injection volumes are easily facilitated with the Aqualog 4.2+ software, which also offers preconfigured blank files.
All data files can be exported with ISO-formatted time-date stamping and user-configurable Sample ID and repeat codes.
All aspects of the Fast-01 hardware control are at your fingertips, with key real-time access features to facilitate the configuration and execution of your batch experiments, as well as priming, cleaning and maintenance.
This study describes the application of simultaneous absorbance and fluorescence excitation-emission matrix (EEM) analysis for the purpose of identification and classification of freshwater planktonic algal species.
Disinfection Byproducts (DBPs) and Precursors in Drinking Water for EPA Compliance
This application note describes the use of the Aqualog for monitoring regulated Dissolved Organic Matter (DOM) and disinfection by-product issues for drinking water treatment.
Global drinking water sources remain prone to carcinogenic petroleum product contaminations due to lack of detection capacity at or before treatment plant intake. The Aqualog A-TEEM method provides a reliable optical detection of these compounds at low quantities discriminating from the highly absorbing and fluorescent backgrounds of natural Dissolved Organic Matter (DOM) components.