Fluorolog-QM

Modular Research Fluorometer for Lifetime and Steady State Measurements

The HORIBA Fluorolog-QM™ series of modular research grade spectrofluorometers is the fourth generation of the world famous HORIBA Fluorolog, with the first Fluorolog introduced by Spex Industries in 1975.

The Fluorolog-QM represents the culmination of decades of HORIBA’s industry-leading experience in development and manufacture of the highest level of spectrofluorometer performance and versatility. The Fluorolog-QM, with its exquisite, optically perfect, all reflective optics, combined with a multitude of light source and detector options, and sample handling accessories, provides the highest sensitivity and greatest versatility of any spectrofluorometer.

The Fluorolog-QM can be enhanced to suit a broad array of luminescence experiments, with the industry’s most extensive list of optional accessories to expand capabilities and performance, to meet all of the most demanding research lab’s experimental needs. And when you purchase a Fluorolog-QM all of these enhancements and accessories can be added to your system at any later time as your needs change, or funds become available.

With thousands operating in universities and research labs around the world, and tens of thousands of publications, Fluorolog has proved itself to be the best choice for the most demanding steady state, time-resolved, TCSPC, PLQY and NIR research.

Segment: Scientific

Division: Fluorescence Spectroscopy

Manufacturing Company: HORIBA Scientific

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Fluorolog-QM Showcase

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4th generation from the Fluorolog series spectrofluorometers

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Spectroscopy in :60 - Up Conversion

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Spectroscopy in :60 - SSTD

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Unique Fluorolog-QM™ Benefits

All reflective optics for perfect performance at all wavelengths
Highest guaranteed sensitivity (more than double that of the Fluorolog-3)
Excellent stray light rejection with extra-large, coma corrected, 350 mm single, or 700 mm double additive monochromators
New software for all steady state and lifetimes experiments, with many new features
Extended wavelength range from Deep UV to NIR
Up to four light source types, and six detectors, can all be connected at once, under computer control, for ultimate lab versatility
World class, plug and play, TCSPC lifetime enhancements, at speeds up to 100 MHz
NIR steady state and phosphorescence lifetime detection to 5,500 nm
Deep UV excitation down to 180 nm

For more features download the Fluorolog-QM brochure.

Optional Pulsed Laser and LED Light Sources

The following specifications are for the standard Fluorolog-QM-75-22 system.
Options and upgrades may be available upon request.

Signal-to-Noise Ratio	(>35,000:1 FSD)
Data Acquisition Rate	1,000,000 points/sec. to 1 point/1000 sec.
Inputs	4 photon counting (TTL); 4 analog (+/- 10 volts); 1 analog reference channel (+/- 10 volts); 2 TTL
Outputs	2 analog (+/- 10 volts); 2 TTL
Emission Range with standard PMT	185 nm to 900 nm (optional to 5,500 nm)
Light Source	High efficiency “ECO” friendly continuous 75 W Xenon arc lamp (Optional 450 W Xenon)
Excitation and Emission Monochromators	700 mm, triple grating, coma-aberration corrected, asymmetrical, excitation or emission optimized, Czerny-Turner design with computer controlled slits at entrance, intermediate plane and exit.
Slits	Computer controlled, continuously adjustable
Excitation Grating	1200 line/mm 300 nm blaze, (Up to two optional gratings can be ordered)
Emission Grating	1200 line/mm 400 nm blaze, (Up to two optional gratings can be ordered)
Wavelength Accuracy	+/- 0.3 nm
Minimum Step Size	0.01 nm (grating dependent)
Standard Detection	Multimode, -20 degrees Celsius cooled, 1600 Volt PMT housing; Multimode: Photon Counting, 3 analog (fast, medium, slow response), direct and Single-Shot Transient Digitizer (SSTD) mode, and Time-Correlated Single Photon Counting (TCSPC), R928P PMT standard (other PMT's available)
R928P PMT Dark Count:	10 counts per second or less
R928P PMT Maximum Linear Count Rate:	10,000,000 counts per second
System Control	Computer interface with FelixFL spectroscopy software
Lifetime Range	5 ps to seconds with appropriate time-resolved accessories

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HORIBA Scientific has a policy of continuous product development, and reserves the right to amend part numbers,
descriptions and specifications without prior notice.

Time-Resolved Electroluminescence with Fluorolog-QM

The Fluorolog-QM’s advanced modularity supports the characterization of electroluminescent materials across a broad time range, from nanoseconds to seconds, without the need for external devices.

Selection Guide for Solid State NIR Detectors on the Fluorolog-QM

This technical note serves as a guideline to selecting the best solid state detector with the Fluorolog-QM modular research spectrofluorometer for a given series of photoluminescence experiments.

Photoluminescence Upconversion with the Fluorolog-QM

The new Fluorolog-QM spectrofluorometers, due to their modularity and advanced software and a universal interface, are an ideal choice for studying multiple aspects of upconversion. This technical note illustrates the use of Fluorolog-QM-75-21 for spectral and time-resolved characterization of these materials.

PLQY in NIR with Fluorolog-QM and K-Sphere

The Fluorolog-QM fluorescence spectrometer with an integrating sphere option is an excellent choice for PLQY measurements in NIR. The K-Sphere is very convenient and easy to use, as it couples directly to the sample compartment optics and allows a use of external light sources, such as DPSS lasers, which can be attached to the front of the sample compartment. The sphere has holders for cuvettes, slides and powders that can be easily interchanged. The results demonstrate very good reproducibility and precision for NIR PLQY measurements spanning almost two orders of magnitude. Based on multiple measurements, the demonstrated PLQY standard deviations range from 1.3% for high QY value (77%) to 6.4% for the QY below 1%.

Kinetic Fluorescence Determination of Vitamin B1

This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution.

Monitoring Culture Medium Conditions During Cell Proliferation Using the Veloci BioPharma Analyzer

Monitoring culture media conditions is critical for optimizing cell growth in the Biopharma industry. The health of cells has drastic impacts on the yield and quality of APIs in regenerative medicine, monoclonal antibody production (mAB), protein synthesis, and many more applications.

Endogenous Skin Fluorescence In Vivo on Human Skin

The fluorescence spectra of intrinsic protein fluorophores have been studied extensively and used in investigating biological events.

Samples can be valuable. Either you don’t want to waste them, or you may not have very much starting material. Biological proteins and enzymes, for example, are often obtained in small volumes and may be expensive.

When a fluorophore absorbs a photon of light, an energetically excited state is formed. The fate of this species is varied, depending upon the exact nature of the fluorophore and its surroundings, but the end result is deactivation (loss of energy) and return to the ground state.