OpenPleX

Manual label-free molecular interaction analysis machine Flexible Research Platform

Surface Plasmon Resonance imaging made easy

Designed to meet the demands of biologists, biochemists and biophysicists, the OpenPlex is a flexible surface plasmon resonance imaging system.
OpenPlex is your companion for the development of label-free and multiplexed bio-assays and molecule detection. It is a robust and compact system designed for simple use and high versatility. Its open format, dedicated sensor chips and manual operation enable numerous types of experiments to be explored without compromise, covering chemistry, biochemistry, physico-chemistry and biomolecular interactions.

Open your research areas with OpenPlex! Choose between three different flow cell configurations without concession:

Normal flow cell for standard surface plasmon resonance imaging experiments
Window flow cell, compatible with fluorescence measurements
Cuvette cell, compatible with electrochemistry measurements

Segment: Scientific

Division: Surface Plasmon Resonance Imaging

Manufacturing Company: HORIBA France SAS

Manual and flexible Surface Plasmon Resonance imaging system for:

Label-free interaction analysis
Real-time monitoring of kinetic curves
Determination of affinity/kinetic parameters
Multiplexed imaging (up to 400 spots)
NEW: Temperature control: 15 - 40 °C

Testimonials

"We are able to generate consistent results that enable us to identify full binding coefficients for multiple candidate aptamer sequences simultaneously. The instrument […] has become one of the most essential tools that we have." Dr. G. Penner, NeoVentures Biotechnologies, Canada

"…an essential tool […] for the characterization of sophisticated ultrasensitive diagnostic platforms integrated with either nanomaterials, modified gold nanostructures or microfluidics”
Prof. Maryam Tabrizian, McGill University, Canada

“The accessibility of the hardware and the flexibility of the software […] allow us to stretch the limits of what can be studied with SPRi. We have been able to modify the flow cell to study bacterial cells and biofilms in unique geometries.”
Dr. E. D. Goluch, Northeastern University, USA

Sample volume: typically 200 μL
Sample concentration: 300 ng/mL (100-1000 kDa) to 10 μg/mL (4-20 kDa)
Sample molecular weight: > 240 Da
Limit of detection: 5 pg/mm²

The Detection of a Low Molecular Weight Enzyme Inhibitor using the OpenPlex™ system

The use of low molecular weight molecules in the industries of drug discovery and agri-food has become increasingly important over the course of a century of drug research and science development in agriculture and in pharmaceutical field.

Real time detection of lymphocytes with SPRi

SPRi Imaging of oligosaccharides proteins interactions

The majority of proteins are glycosylated: they possess oligosaccharide chains and are hence termed glycoproteins. Oligosaccharides, also called carbohydrate or sugar, are often quite large (as large as some protein domains for example) and they have many functions in molecular interactions.

Protein-Peptide interactions studies with SPRi

Description of the advantages of combining the use of peptide chips with direct label-free detection as achieved by SPR imaging (SPRi).

Interaction between immobilized peptides and protein from sera on Cystamine / Glutaraldehyde functionalized biochip

Our SPRi technology (Surface Plasmon Resonance imaging), is able to measure multiplexed label-free biolo-gical interactions in a complex media such as serum.We will demonstrate the ability to monitor specific interactions between immobilized peptides and proteins issued from non purified immune sera, using a cystamine/glutaraldehyde SAM surface chemistry on the bio-chip surface. We chose to illustrate this ability by monitoring the interaction between an ovalbumin peptide fragment and an ovalbumin immunized serum.

HORIBA provides the pharmaceutical industry, biotech companies and academic research laboratories with high performance instrumentation based on SPRi technology (Surface Plasmon Resonance imaging). To demonstrate the power of this technology, we carried out an experiment based on an antibody-antigen interaction. Original surface chemistry combined with an electrochemical process allows the rapid coupling of biomolecules to the gold layer (polypyrrole co-polymerisation) on the top of a glass prism.

Size: 2.34 MB

OpenPlex Brochure