DeltaFlex

TCSPC/MCS Fluorescence Lifetime System

Ultimate TCSPC/MCS Performance and Flexibility for Lifetimes From 5 Picoseconds to Seconds

The HORIBA DeltaFlex™ system is the Time-Correlated Single Photon Counting (TCSPC) system of choice for the measurement of fluorescence lifetimes from picoseconds to seconds. Taking advantage of more than 45 years of TCSPC innovation, it is a complete fluorescence/phosphorescence system designed and optimized with innovative pulsed lasers and LEDs, timing electronics, detectors and sample handling components, all powered by HORIBA’s highly intuitive and extremely powerful EzTime™ touchscreen software interface.

Segment: Scientific

Division: Fluorescence Spectroscopy

Manufacturing Company: HORIBA Scientific

Unique Benefits from a Lifetime in Fluorescence

Flexibility to meet any requirement
Better user experience with automated component recognition and control
Extensive choice of sources and detectors to meet all wavelength and lifetime requirements
EzTime intuitive, automated, touchscreen software

DeltaFlex: Flexibility is in the name, and flexibility is in the design

The modular DeltaFlex system is comprised of a choice of the following main components:

Optical configuration
Choose from filters or monochromators and single detector (L format) or simultaneous dual detector (T Format)
Excitation sources
Choose from a plethora of pulsed lasers and LED sources
Detection modules
Choose from a wide range of dedicated TCSPC detectors depending on your emission wavelength range, lifetime requirements and budget
Timing electronics
Choose from standard or high resolution electronic interfaces depending on your lifetime requirements

All DeltaFlex systems are controlled using EzTime software which provides for full instrumentation control and acquisition, as well as a comprehensive suite of data analysis modules for fluorescence and phosphorescence lifetime determination, decay associated spectra (DAS), time-resolved anisotropy and uncorrected steady state emission spectra (if equipped with a scanning emission monochromator).

Optional Pulsed Laser and LED Light Sources

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DeltaDiode™ General Specifications

DeltaDiode Type	Pulse Duration	Repetition Rate	Available Wavelengths
DeltaDiode Laser (Denoted with an “L” on part number)	35 to 200 ps	10 kHz to 100 MHz	375 to 1,310 nm
DeltaDiode LED (No “L” on part number)	750 to 950 ps	10 kHz to 25 MHz	265 to 455 nm

DeltaDiode Controller Specifications

DD-C1 Controller

Function	Specification
Repetition Rates	10 kHz, 20 kHz, 50 kHz, 100 kHz, 250 kHz, 500 kHz, 1 MHz, 2 MHz, 4 MHz, 5 MHz, 8 MHz, 10 MHz, 16 MHz, 20 MHz, 25 MHz, 50 MHz, 80 MHz, 100 MHz, or Trigger Input (single-shot to 50 MHz). Subject to attached head
Trigger Input	Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω, 20 ns minimum spacing
Sync Outputs	Simultaneous output of NIM-compatible (-0.8 Vpp 50 Ω) and TTL-compatible (+2 Vpp 50 Ω), automatic width selection 4-15 ns nominal
Sync Delay Control	Adjustment of sync output pulse timing in range -10 ns to +10 ns nominal in 1 ns steps, uncalibrated
Fast Gate Input	Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω. Selectable Inhibit/Enable modes
Slow Gate Input	Pulse amplitude +2 V to +5 V. 10 kΩ. Selectable Inhibit/enable modes. Operates in Pulsed and CW modes
Interlock	2-pin connector (included). Contacts must be short-circuited to enable emission
Connection to Head	1.5 m cable (included)
User Interface	LCD display (stand-alone operation) or software (PC control)
PC interface	USB 2.0 with integral hub for downstream connection to other USB peripherals (cable to host PC and software supplied)
Power Requirement	90 V to 250 V AC, 50/60 Hz, 100 VA
Operating Temperature	+15˚ C to +30˚ C (ambient)
Weight & Dimensions	3.1 kg, 234 x 255 x 92 mm

Solas MOFA Fiber Laser Specifications

Parameter	Solas 355L	Solas 532L	Solas 1064L
Power	> 7 mW at 80 MHz	> 30 mW at 80 MHz	> 300 mW at 80 MHz
Power Stability	0.5% RMS over 10 min
Pulse duration	80 to 100 ps (FWHM)
Spectral Width	< 0.2 nm		< 0.4 nm
Resolution Rate	Pulse on demand to 100 MHz
Pointing Stability	< 1 μrad
Polarization Extinction Ratio	> 20 dB
Beam Quality	Multi-mode fiber output	Circular TEM00 beam

SpectraLED Specifications

	Pulse Duration	Repetition Rate	Available Wavelengths
SpectraLED	< 250 ns to > 10 s	0.1 to 100 kHz	265 to 1,275 nm

SpectraXE Specifications

	Pulse Duration	Repetition Rate	Available Wavelengths
SpectraXE	0.4 μs	0.1 Hz to 80 Hz	185 to 2,000 nm

HPPD Specifications

Model	Wavelength Range	Temporal Response (IRF FWHM)	Dark Count	Quantum Efficiency
HPPD-650	220 to 650 nm	50 ps	< 100 cps	28% (340 nm)
HPPD-720	300 to 720 nm	120 ps	< 1000 cps	47% (530 nm)
HPPD-860	220 to 860 nm	50 ps	< 200 cps	23% (280 nm)
HPPD-870	300 to 870 nm	130 ps	< 500 cps	26% (630 nm)
HPPD-890	380 to 890 nm	160 ps	< 1000 cps	16% (630 nm)

MCP-PMT Detectors

	HPPD-860 COOLED	MCP-PMT (R3809-50)
Typical IRF FWHM at 400 nm	40-45 ps	40-45 ps
Shortest Measurable Lifetime	40-45 ps	5 ps
Wavelength Response	220-860 nm	160-850 nm
Robust for Steady State Spectra	Yes	No
Amplifier + CFD	Integrated (no cable)	External
High Voltage Bias	Integrated (no cable)	External
Compatible with Phos and Steady State	Yes	No (requires second detector)
Temperature Control	Integrated TEC (air-cooled)	External (water-cooled)
Dark Count Rate (Cooled)	< 200 cps	< 20 cps
PC Interface	N/A	N/A

NIR TCSPC Specifications

Model	Sensor	Wavelength Range	Temporal Response	Dark Count	Cooling
NIR-R4	R5509-43 PMT	300 to 1,400 nm	1.5 ns (TTS)	< 25,000 cps	Liquid nitrogen
NIR-R7	R5509-73 PMT	300 to 1,700 nm	1.5 ns (TTS)	< 250,000 cps	Liquid nitrogen
NIR-H2	H10330-25 PMT	950 to 1,200 nm	400 ps (TTS)	< 2,500 cps	Thermo-electric
NIR-H4	H10330-45 PMT	950 to 1,400 nm	400 ps (TTS)	< 25,000 cps	Thermo-electric
NIR-H7	H10330-75 PMT	950 to 1,700 nm	400 ps (TTS)	< 250,000 cps	Thermo-electric
NIR-S1	Count-100N SPAD	400 to 1,000 nm	< 3 ns (TTS)	100 cps	None

FiPho TCSPC Electronics Specifications

Specifications	FiPho	FiPho-HR
Full Detectable TCSPC Lifetime Range	<20 ps to 30 sec	5 ps to 30 sec
TCSPC Converter Type	Digital TDC	Digital TDC and Analog TAC
TCSPC Bin Width	<15 ps	~ 250 fs
Phosphorescence Mode	MCS	MCS
Independent Stop Channels	1 to 4	1 to 4
Photon Streaming	Included	Included
FLIM Capable	Yes	Yes

Detailed FiPho Electronics Specifications

Specifications	FiPho (TDC, MCS)	FiPho-HR (TDC, TAC, MCS)
Full Detectable Lifetime Range	<20 ps to 30 sec	5 ps to 30 sec
TCSPC Time Range	<2 ns to 55 μs	<2 ns to 55 μs
Deadtime	5 ns	5 ns
TCSPC Bin Width	<15 ps	~ 250 fs
Electronics Jitter (FWHM)	30 ps	< 10 ps
TCSPC Histogram Size	Up to 16k	Up to 64k
Histogram Bin Depth	32 bit	32 bit
Independent Stops	1 to 4	1 to 4
Maximum Start Rate	100 MHz	100 MHz
Maximum Stop Rate	40 Mcps	40 Mcps
Operating Mode	Automatic Forward Timing	Automatic Forward or Reverse timing
Streaming Mode	Photon Streaming (Time-Tag)	Photon Streaming (Time-Tag)
MCS Bin Width	5 ns	5 ns
MCS Time Range	< 2.5 μs to 330 seconds	< 2.5 μs to 330 seconds
Maximum MCS Histogram Size	64k	64k
Acquisition and Analysis Macro Scripting	Yes	Yes
PC Interface	USB 3.0	USB 3.0
Software	EzTime, EzTime Image	EzTime, EzTime Image

HORIBA Scientific has a policy of continuous product development, and reserves the right to amend part numbers, descriptions and specifications without prior notice.

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